What Is Biology & Characteristics of Life
Level 4: Application (Novel Problems)
Time: 60 minutes | Total marks: 50
Question 1 — The Mystery Object (10 marks)
A probe on a distant moon collects a spherical grey object. Over 30 days, scientists record:
- The object slowly increases in diameter from 4 cm to 6 cm.
- It absorbs a nearby liquid, releasing a gas as it does so.
- When the surrounding temperature rises, its internal temperature rises identically and does not stabilise.
- Splitting it in half produces two smaller objects that each continue to grow.
- It shows no response when poked, shaken, or exposed to light.
(a) Using the seven characteristics of living things, evaluate whether this object is living. Reference at least four characteristics explicitly, stating for each whether the evidence supports "living." (6)
(b) State your overall verdict (living / non-living / once-living / undetermined) and justify it. (2)
(c) Design ONE additional test the scientists could run to reduce the uncertainty, and state what result would support "living." (2)
Question 2 — Metabolism & Homeostasis in Context (10 marks)
A patient's blood glucose is monitored after a meal. Values (mmol/L): time 0 = 5.0, +30 min = 8.5, +60 min = 7.0, +90 min = 5.4, +120 min = 5.0.
(a) State whether glucose homeostasis appears to be functioning, and use two data points to justify. (3)
(b) After the meal, the liver converts excess glucose into glycogen for storage. Classify this reaction as anabolic or catabolic and justify using the definition. (3)
(c) During intense exercise later, muscle cells break glucose down to release energy. Classify this reaction and explain how it differs energetically from the process in (b). (2)
(d) Explain why maintaining a stable internal glucose level is an example of homeostasis rather than merely metabolism. (2)
Question 3 — Levels of Organisation & Emergent Properties (10 marks)
A student writes this list of biological components observed in a pond ecosystem, out of order: muscle tissue, oxygen atom, a single frog, all the frogs in the pond, a heart, the whole pond community, a nerve cell.
(a) Arrange these seven items from smallest to largest in the correct sequence of biological organisation. (4)
(b) Define "emergent property" and give two examples using items from the list, showing what property emerges at each transition. (4)
(c) The heart can pump blood, but an isolated heart-muscle cell cannot pump blood. Explain what this demonstrates about biological organisation. (2)
Question 4 — Experimental Design (12 marks)
A gardener claims: "Plants watered with sugar-water grow taller than plants watered with plain water." She sets up 20 identical seedlings, 10 per group.
(a) Write a testable hypothesis for this investigation. (2)
(b) Identify the independent variable, the dependent variable, and TWO variables that must be controlled. (4)
(c) Explain the role of the plain-water group in this experiment. (2)
(d) The gardener uses only ONE seedling per group instead of ten. Explain why this weakens her conclusions. (2)
(e) State whether the gardener's claim, once repeatedly supported by evidence, would become a hypothesis, a theory, or a law. Justify. (2)
Question 5 — Data, Units & Graphs (8 marks)
A bacterial culture is measured. Population (cells): hour 0 = 200; hour 1 = 400; hour 2 = 800; hour 3 = 1600; hour 4 = 3200.
(a) Describe the growth pattern shown and state what you would expect a line graph of this data to look like. (2)
(b) Calculate the total increase in cell number from hour 0 to hour 4. (2)
(c) A single bacterium has a length of 0.000002 m. Express this length in micrometres () using the correct metric prefix. (2)
(d) If measurements were taken every 30 minutes instead of every hour, state the SI unit and value in seconds of that time interval. (2)
End of paper
Answer keyMark scheme & solutions
Question 1 (10 marks)
(a) Award 1 mark per characteristic correctly discussed (max 6 = up to 4 characteristics with evidence + reasoning). Expected:
- Growth — diameter 4→6 cm: supports living (increase in size). (1)
- Nutrition/Metabolism — absorbs liquid, releases gas suggests chemical reactions/gas exchange: supports living. (1)
- Homeostasis — internal temp tracks external, does NOT stabilise: argues against living (no self-regulation). (1)
- Reproduction — splitting produces growing copies resembles asexual reproduction: supports living. (1)
- Responsiveness/irritability — no response to poke/shake/light: argues against living. (1)
- (Movement/excretion may be mentioned — gas release = excretion, supports.) (1)
(b) Verdict: Undetermined / most likely non-living (2). Justify: it shows some criteria (growth, apparent reproduction) but crucially FAILS homeostasis and responsiveness — a genuine living thing should display all seven; growth + splitting can occur in non-living crystals/chemical systems. (Full marks for consistent verdict backed by the missing criteria.)
(c) 1 mark for valid test + 1 for expected "living" result. E.g. test for response to a stimulus over time / offer a nutrient and check for controlled uptake and metabolic waste / check for cells under a microscope. Living result = shows response OR contains cells as basic unit of life.
Question 2 (10 marks)
(a) Yes, functioning (1). Glucose rose to 8.5 at +30 (2) then returned to baseline 5.0 by +120 min — the return to the original set-point demonstrates regulation (1).
(b) Anabolic (1). Glycogen is a larger molecule built from smaller glucose units (1); anabolism = building complex molecules from simpler ones, requiring/storing energy (1).
(c) Catabolic (1). Catabolism breaks larger molecules into smaller ones and releases energy, whereas the anabolic reaction in (b) consumes/stores energy (1).
(d) Metabolism is simply the sum of chemical reactions; homeostasis is the maintenance of a stable internal set-point (1) — here glucose is actively returned toward ~5.0 mmol/L despite the disturbance from the meal, showing regulation, not just reaction (1).
Question 3 (10 marks)
(a) Smallest → largest (award 4 for fully correct; –1 per misplaced): oxygen atom → nerve cell → muscle tissue → heart (organ) → single frog (organism) → all frogs in pond (population) → whole pond community. (4)
(b) Emergent property = a new characteristic that appears at a higher level of organisation that is not present in the individual parts alone (2). Two valid examples (1 each), e.g.:
- Cells → tissue: coordinated contraction (muscle tissue contracts, a lone property vs single cell).
- Organs → organism: the whole frog can move, feed and reproduce — properties no single organ has.
(c) Demonstrates emergent properties / that the whole is more than the sum of parts (1): pumping arises from the organised arrangement of many cells into an organ, not from any single cell (1).
Question 4 (12 marks)
(a) E.g. "Seedlings watered with sugar-water will grow taller than seedlings watered with plain water." Must be testable & predictive. (2)
(b) Independent variable = type of water (sugar vs plain) (1); Dependent variable = plant height/growth (1); Controlled variables (2, any two): light, temperature, volume of water, soil type, seedling species/initial size. (2)
(c) The plain-water group is the control (1): it provides a baseline/comparison so any difference in growth can be attributed to the sugar rather than to watering itself (1).
(d) With n=1, results may be due to individual variation/chance, not the treatment (1); no repetition means no reliability and no way to spot anomalous results (1).
(e) A theory (1): a well-supported explanation backed by repeated evidence — it is not a mathematical law, and it is more than a single untested hypothesis (1). (Accept reasoned discussion that a claim/prediction alone is a hypothesis.)
Question 5 (8 marks)
(a) Population doubles each hour → exponential/geometric growth (1); a line graph would show an upward-curving (J-shaped) exponential curve, getting steeper over time (1).
(b) Total increase = 3200 − 200 = 3000 cells (2).
(c) (since m). (2)
(d) 30 minutes = 30 × 60 = 1800 s; SI unit = second (s) (2).
[
{"claim": "Total cell increase hour0 to hour4 is 3000", "code": "result = (3200 - 200 == 3000)"},
{"claim": "0.000002 m equals 2 micrometres", "code": "result = (0.000002 / 1e-6 == 2)"},
{"claim": "30 minutes equals 1800 seconds", "code": "result = (30 * 60 == 1800)"},
{"claim": "Bacterial population doubles each hour (200,400,800,1600,3200)", "code": "vals=[200,400,800,1600,3200]; result = all(vals[i+1]==2*vals[i] for i in range(len(vals)-1))"}
]